| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.052489 |
| Chromosome: | chromosome 14 |
| Location: | 3556369 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631000 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTGATGATAAGGACTGCCTGACATATG |
| Internal bar code: | CACTCGCAACTCGCCTCGCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 499 |
| LEAP-Seq percent confirming: | 99.7446 |
| LEAP-Seq n confirming: | 7030 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTGCATTCGACTACATT |
| Suggested primer 2: | TTGAGGAGATCCTGGAGTCG |