| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.052496 |
| Chromosome: | chromosome 3 |
| Location: | 3149453 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g165150 | (1 of 31) IPR003594 - Histidine kinase-like ATPase, C-terminal domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTCATCGCACTATGCTACATGCTGTGC |
| Internal bar code: | TGTTTAGGCGGCAGCCCACCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 747 |
| LEAP-Seq percent confirming: | 98.5823 |
| LEAP-Seq n confirming: | 3616 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCGGTTCTCTGTTCAAGG |
| Suggested primer 2: | GCGTAGCAAGAAGGGACAAG |