Insertion junction: LMJ.RY0402.052513_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.052513
Chromosome:chromosome 4
Location:3232166
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g226550 ARS11 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCCCAGACCCCACAGCCACCCCCAGTTC
Internal bar code:CTAGGAGGTCCGTTTGTGAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:338
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TCTTTCAATTGCCTCGTCCT
Suggested primer 2:AAACCCCTGGCCAATTTTAC