Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.052539 |
Chromosome: | chromosome_1 |
Location: | 2903806 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g017650 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATGATTGCAGTCGTTGTAGCGGTTGAGAAA |
Internal bar code: | TGGACTGACCTTCGCCCTAGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 842 |
LEAP-Seq percent confirming: | 99.7242 |
LEAP-Seq n confirming: | 1808 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATTGTCTAATCAGCCGTGCC |
Suggested primer 2: | CTCGCCCAGCTTACTTCAAC |