| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.052540 |
| Chromosome: | chromosome_6 |
| Location: | 1745526 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g261900 | FAP71 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCTGCATTTGTGTCTTCCCTATGTGGGCGA |
| Internal bar code: | AAGCATTTGCCCTTTCGGTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1348 |
| LEAP-Seq percent confirming: | 99.6175 |
| LEAP-Seq n confirming: | 5469 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGACTGCGAGGTGAACAA |
| Suggested primer 2: | CGCAGAAGCTGTCTGTCAAG |