| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.052546 |
| Chromosome: | chromosome 8 |
| Location: | 8589 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358200 | ACV4 | protein kinase; (1 of 6) PTHR23257//PTHR23257:SF529 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATCAACCGCTGTGTTGACTTACGGGTGC |
| Internal bar code: | CTCTACCACATTGACCGGCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 907 |
| LEAP-Seq percent confirming: | 97.1717 |
| LEAP-Seq n confirming: | 1924 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCGTAGTTGAGGCCCAT |
| Suggested primer 2: | GCCCAAGAACAAAGAAGCTG |