| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.052565 |
| Chromosome: | chromosome 17 |
| Location: | 3257427 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g722851 | (1 of 6) 1.1.1.100 - 3-oxoacyl-[acyl-carrier-protein] reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGCCGCTCTCTCTGGTGTTAGCAGCGC |
| Internal bar code: | GCAGTCGAGGGGTGCCACAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 206 |
| LEAP-Seq percent confirming: | 8.4077 |
| LEAP-Seq n confirming: | 179 |
| LEAP-Seq n nonconfirming: | 1950 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACACACTCCAGCTCCAA |
| Suggested primer 2: | TCAGCAGCCACATCCTGTAG |