| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.052610 |
| Chromosome: | chromosome 7 |
| Location: | 4029224 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g340100 | MOT13 | Predicted protein; (1 of 5) PF07496 - CW-type Zinc Finger (zf-CW) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCGCTGCTGCTGGGACCGCGGTCCCTA |
| Internal bar code: | CGATCGTTGATAGATTATTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 50.4621 |
| LEAP-Seq n confirming: | 2184 |
| LEAP-Seq n nonconfirming: | 2144 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCATCCTATCCGCAGCAG |
| Suggested primer 2: | CTAGCCTGTTCCGCAATCTC |