| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.052612 |
| Chromosome: | chromosome 4 |
| Location: | 1286862 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214800 | VMPL1,VMP6 | (1 of 10) PF00957 - Synaptobrevin (Synaptobrevin); Endosomal R-SNARE protein, VAMP-like family (R.III) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCTCATGAGGTCGGACACTTGGACCCGA |
| Internal bar code: | GGGCGCCCGGTAACGAGCTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 999 |
| LEAP-Seq percent confirming: | 99.3119 |
| LEAP-Seq n confirming: | 1732 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGGACGTGTGATGGCAGT |
| Suggested primer 2: | TGACGTACCTCCACCAACAA |