Insertion junction: LMJ.RY0402.052615_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.052615
Chromosome:chromosome 10
Location:2989950
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g440800 (1 of 88) IPR011333 - POZ domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGGCAGTGGTGGAGGCGGCAGTAGCAGT
Internal bar code:TGCGATCTTTTCGCTCAAGCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:616
LEAP-Seq percent confirming:99.4822
LEAP-Seq n confirming:1345
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCTCATCCTCACCCTGAC
Suggested primer 2:TCCGAGGACGAGGAAGACTA