Insertion junction: LMJ.RY0402.052618_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.052618
Chromosome:chromosome 1
Location:1504540
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g007737 FBT1 Folate/pteridine transporter 1; (1 of 1) IPR004324//IPR011701//IPR020846 - Folate-biopterin transporter // Major facilitator superfamily // Major facilitator superfamily domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTAACTTGACTAACTTCGTTTTCCGCATG
Internal bar code:TGTATGGGTTTATAACTCGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGTACTTCCTGTCCTTGCCC
Suggested primer 2:GCTGTCAGAGTTGGGGATGT