| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.052622 |
| Chromosome: | chromosome 2 |
| Location: | 3579476 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095134 | (1 of 2) 2.1.1.9 - Thiol S-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTATACCTCTGCACGCTTGTATTGTATGT |
| Internal bar code: | GCGCAGGCCGTGTTGACAAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 635 |
| LEAP-Seq percent confirming: | 69.3005 |
| LEAP-Seq n confirming: | 1912 |
| LEAP-Seq n nonconfirming: | 847 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTTACAGCGGCATAACA |
| Suggested primer 2: | ATGTCAGTTGCTTGGGGAAC |