Insertion junction: LMJ.RY0402.052632_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.052632
Chromosome:chromosome 2
Location:3275443
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g095081 (1 of 1) PF09295 - ChAPs (Chs5p-Arf1p-binding proteins) (ChAPs) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCTCCCAACCCTCGGAACTGCGCCGCCG
Internal bar code:TTCCCTCCGCAGCTGTTTTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:64
LEAP-Seq percent confirming:95.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCCAGGTGTGGACTTTGAGT
Suggested primer 2:ACTGCCGGTATTGCTGCTAC