| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.052632 |
| Chromosome: | chromosome 5 |
| Location: | 861426 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g247650 | (1 of 32) IPR000536 - Nuclear hormone receptor, ligand-binding domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGACGGGCCCAAGGCCAAGAAAACCAA |
| Internal bar code: | AATTATTAACCGTCTGCAGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 99.4331 |
| LEAP-Seq n confirming: | 4736 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGAGCTATCAGGCTCCATT |
| Suggested primer 2: | CGCCATGATCAATCTCAATG |