| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.052642 |
| Chromosome: | chromosome 1 |
| Location: | 1753756 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g009400 | NSS2 | (1 of 2) PTHR11819//PTHR11819:SF12 - SODIUM/SOLUTE SYMPORTER // HIGH-AFFINITY CHOLINE TRANSPORTER 1; Sodium:solute symporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGGGCGGCAGGTGGCGATGGGCACAGT |
| Internal bar code: | ACGCCGGTTTGGGGGATCCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 589 |
| LEAP-Seq percent confirming: | 99.7383 |
| LEAP-Seq n confirming: | 11433 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGCACCTACCCTCCAAA |
| Suggested primer 2: | GTTGGCTGCTGGAAATGAAT |