Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.052642 |
Chromosome: | chromosome_1 |
Location: | 1753756 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g009400 | NSS2 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGGTGGGCGGCAGGTGGCGATGGGCACAGT |
Internal bar code: | ACGCCGGTTTGGGGGATCCGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 589 |
LEAP-Seq percent confirming: | 99.7383 |
LEAP-Seq n confirming: | 11433 |
LEAP-Seq n nonconfirming: | 30 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAATGCACCTACCCTCCAAA |
Suggested primer 2: | GTTGGCTGCTGGAAATGAAT |