| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.052643 |
| Chromosome: | chromosome 2 |
| Location: | 2460099 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091900 | FAP301 | Flagellar Associated Protein 301 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCGCGAGGGCGACATGGAGCCGGGCATG |
| Internal bar code: | GAGGATACGTGAGCAGGGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 672 |
| LEAP-Seq percent confirming: | 99.6842 |
| LEAP-Seq n confirming: | 947 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACCCTGCTTTGTTCCTC |
| Suggested primer 2: | CTATCCTACCTCGCAGTCGC |