| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.052648 |
| Chromosome: | chromosome 13 |
| Location: | 3966008 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g590905 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTCGCCCTACACTTGCAGCTTTTCTCCA |
| Internal bar code: | TATGCTCACATGCACAGCCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 302 |
| LEAP-Seq percent confirming: | 97.992 |
| LEAP-Seq n confirming: | 488 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGGTCGTGCGTAATGCAG |
| Suggested primer 2: | TGAAATGCATAATCAGGGCA |