Insertion junction: LMJ.RY0402.052658_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.052658
Chromosome:chromosome 8
Location:4088870
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g379550 MFT11,PHT4,PHT4-4,PHT4D Sodium-dependent phosphate transporter, major facilitator superfamily; (1 of 4) K08193 - MFS transporter, ACS family, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), other (SLC17A) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGGCCTGTTCATTCTGCTCACCTGGATG
Internal bar code:TGCAGATATGCTGGCGCGAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:775
LEAP-Seq percent confirming:99.5129
LEAP-Seq n confirming:9398
LEAP-Seq n nonconfirming:46
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGGAATCCTAACACGCTG
Suggested primer 2:CGAAATCACACCACCACTTG