| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.052662 |
| Chromosome: | chromosome 4 |
| Location: | 2349153 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220825 | (1 of 3) PF10601 - LITAF-like zinc ribbon domain (zf-LITAF-like) | CDS/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAGGAGCCTCACACTCACAGGCTAGAAA |
| Internal bar code: | GTGGCTCACTTGCGTGCTGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 324 |
| LEAP-Seq percent confirming: | 99.8211 |
| LEAP-Seq n confirming: | 1116 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGAAGGTAAAACCTGTCA |
| Suggested primer 2: | ATGCAGGTCCCTCGTGTAAC |