| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.052703 |
| Chromosome: | chromosome 7 |
| Location: | 2386969 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g328550 | VPS18 | Subunit of the VPS-C complex; (1 of 1) PF05131 - Pep3/Vps18/deep orange family (Pep3_Vps18) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAGCTTGCGCCGCAGGGCGTCGTCCTGG |
| Internal bar code: | GCCGCCGGTGCGGATGGAGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 277 |
| LEAP-Seq percent confirming: | 82.3129 |
| LEAP-Seq n confirming: | 121 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCAAAGATGGTGCAGAGA |
| Suggested primer 2: | GGCGTCCTTGCAAGACTAAG |