| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.052704 |
| Chromosome: | chromosome 14 |
| Location: | 1434824 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617750 | PDE1,PDE3 | (1 of 4) K13293 - cAMP-specific phosphodiesterase 4 (PDE4); 3'%252C5'-cyclic-nucleotide phosphodiesterase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCAGAGCCTCCAGCAACTCCTTGTTGCG |
| Internal bar code: | TACGCCGCCGAGACACTCTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 849 |
| LEAP-Seq percent confirming: | 99.7466 |
| LEAP-Seq n confirming: | 3542 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGCTTACTGGACTCATTG |
| Suggested primer 2: | GTCAGGTTGAGGGGTTCGTA |