| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.052712 |
| Chromosome: | chromosome 12 |
| Location: | 2700983 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503750 | FMO14 | (1 of 5) 1.14.13.114 - 6-hydroxynicotinate 3-monooxygenase / HNA-3-monooxygenase; Flavin-containing monooxygenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGACTGACGACGCGCTTCCCGGCCTGCA |
| Internal bar code: | TTTCAGCGATAACGGTTAATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 335 |
| LEAP-Seq percent confirming: | 99.4581 |
| LEAP-Seq n confirming: | 2019 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGATTAAGGGAGCCCATC |
| Suggested primer 2: | CTCGGCTACCTCTCCTTGTG |