Insertion junction: LMJ.RY0402.052718_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.052718
Chromosome:chromosome 2
Location:3240914
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095076 ROC39,MFT10 Major facilitator superfamily transporter; (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGAGTGCCGCTGCGCTGAACGCTCTGAGC
Internal bar code:AGTCAGAGGGCCCGTCCTCCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:90
LEAP-Seq percent confirming:99.8374
LEAP-Seq n confirming:614
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTACGAAGGAGTGCCCAT
Suggested primer 2:GCTTCTGCTCCACAGCCTAC