| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.052718 |
| Chromosome: | chromosome 3 |
| Location: | 9042348 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g210513 | (1 of 2) K05894 - 12-oxophytodienoic acid reductase (OPR) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGACATAACGCGCGTGCCTACAAATCCT |
| Internal bar code: | GAGACTGACTTCCACGGGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 717 |
| LEAP-Seq percent confirming: | 97.8238 |
| LEAP-Seq n confirming: | 2787 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCTGTTCCTGATCGTTTG |
| Suggested primer 2: | GACGTTTCACTGACGAGGGT |