| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.052737 |
| Chromosome: | chromosome 6 |
| Location: | 2018954 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g264250 | FAO6 | FAD-dependent oxidoreductase; (1 of 3) 5.5.1.19 - Lycopene beta-cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGAGGAGGTAACGACATGTGTCAGAGAG |
| Internal bar code: | GCTATTGATGTGGCCCACCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 679 |
| LEAP-Seq percent confirming: | 99.6993 |
| LEAP-Seq n confirming: | 6632 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGGTTGTATTCGTGCTTC |
| Suggested primer 2: | ACCGCAGTAGTCAATGGGAC |