| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.052746 |
| Chromosome: | chromosome 7 |
| Location: | 4547239 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g343750 | (1 of 110) 3.4.19.12 - Ubiquitinyl hydrolase 1 / Ubiquitin thiolesterase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGAGGAGCGGCAACCAGGTGCGACTCCG |
| Internal bar code: | TCTCCTTCTCCTCGTATTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 714 |
| LEAP-Seq percent confirming: | 99.115 |
| LEAP-Seq n confirming: | 224 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCTTTTGTGGCAAGTCCC |
| Suggested primer 2: | CACAAGGGAGCTCAAGGAAG |