Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.052760 |
Chromosome: | chromosome_17 |
Location: | 3201544 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g722150 | PKS3 | Type III polyketide synthase | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TCATTCCACAGCACAGTACCCTCCCCGCGC |
Internal bar code: | GTCCTAGTGAGGAGCCTCGTGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1205 |
LEAP-Seq percent confirming: | 78.1575 |
LEAP-Seq n confirming: | 526 |
LEAP-Seq n nonconfirming: | 147 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACTTGCATGGTGGGTACTG |
Suggested primer 2: | ATGAGGAAACAGGCTGTGCT |