Insertion junction: LMJ.RY0402.052762_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.052762
Chromosome:chromosome 14
Location:1544490
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g618600 MRP7 ABC transporter, multidrug resistance associated protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCGGTGCGAGTCGAAGCGAAGCATGCGC
Internal bar code:TCGAGCGAGGAATCTCGGAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:336
LEAP-Seq percent confirming:88.2979
LEAP-Seq n confirming:332
LEAP-Seq n nonconfirming:44
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TCAAGCTCACCACCATCATC
Suggested primer 2:AAACCGAACAGGAGATGGTG