| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.052816 |
| Chromosome: | chromosome 17 |
| Location: | 3387724 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723900 | FAP131,ROC103 | Flagellar Associated Protein 131 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCCGGAGAACTATGGGCCGCTCATACGC |
| Internal bar code: | GCTGCGCCCGATTGCCGTGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 896 |
| LEAP-Seq percent confirming: | 99.3133 |
| LEAP-Seq n confirming: | 7521 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGGAGATAACAGTCCTGG |
| Suggested primer 2: | CTGGAGCGTTAGCATGAACA |