| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.052869 |
| Chromosome: | chromosome 10 |
| Location: | 6422581 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465822 | (1 of 20) IPR010095//IPR013083 - Transposase IS605, OrfB, C-terminal // Zinc finger, RING/FYVE/PHD-type | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGTGCCCCAGCCAACAATGACCTCCTC |
| Internal bar code: | GGGAACCAGTAGCTGGCATAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 722 |
| LEAP-Seq percent confirming: | 35.611 |
| LEAP-Seq n confirming: | 985 |
| LEAP-Seq n nonconfirming: | 1781 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGAGGGTAGAGTGAGTGA |
| Suggested primer 2: | GGGGAGGAGTGTTTATGGGT |