Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.052886 |
Chromosome: | chromosome_12 |
Location: | 4122160 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g518050 | FAP261 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCACCCCGCGAGCGAGCGCGGCACATCAC |
Internal bar code: | TCGACCCACACAAGCACCATGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 361 |
LEAP-Seq percent confirming: | 96.5359 |
LEAP-Seq n confirming: | 1477 |
LEAP-Seq n nonconfirming: | 53 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGAGTACCTGAGGCTTCGC |
Suggested primer 2: | TGACAGCCTGATGCTCATTC |