Insertion junction: LMJ.RY0402.052918_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.052918
Chromosome:chromosome 3
Location:4886095
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g179400 MFT17 Major facilitator superfamily transporter; (1 of 22) IPR011701//IPR020846 - Major facilitator superfamily // Major facilitator superfamily domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTCCATTTGCAATTGGGTCAATCACATT
Internal bar code:CTTTTGGAGGCAGCGTTCGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:856
LEAP-Seq percent confirming:99.3242
LEAP-Seq n confirming:8672
LEAP-Seq n nonconfirming:59
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCGACGAGGAAGAGGTGA
Suggested primer 2:TTTACAATTCGCCGAACTCC