| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.052932 |
| Chromosome: | chromosome 3 |
| Location: | 7623198 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204450 | (1 of 9) IPR017896 - 4Fe-4S ferredoxin-type, iron-sulphur binding domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAAACAACTCTCACAATGTACAAGACGA |
| Internal bar code: | ATGGCACGGGGGTTTGATATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 111 |
| LEAP-Seq percent confirming: | 55.9928 |
| LEAP-Seq n confirming: | 313 |
| LEAP-Seq n nonconfirming: | 246 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTTCACCTTCAATTCAT |
| Suggested primer 2: | GCAACTGGGGTGTATCGACT |