| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.052934 |
| Chromosome: | chromosome 17 |
| Location: | 5912473 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g740050 | (1 of 2) IPR003582//IPR011050//IPR019316 - ShKT domain // Pectin lyase fold/virulence factor // G8 domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACTGTTTATTGTGCGAACATTCATCCGA |
| Internal bar code: | TAATCCTGCGTAACCTGCGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1284 |
| LEAP-Seq percent confirming: | 99.6089 |
| LEAP-Seq n confirming: | 8914 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGAATGGGCATGCTGTGT |
| Suggested primer 2: | GTGCCGTGGTTCTCAAGAGT |