| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.052944 |
| Chromosome: | chromosome 3 |
| Location: | 2459666 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159400 | (1 of 2) 2.7.7.73 - Sulfur carrier protein ThiS adenylyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCGCCTCCAGGTCCGCCTGGGCCGTGG |
| Internal bar code: | AGTGCTCGTTTTAGGAGGCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 332 |
| LEAP-Seq percent confirming: | 99.6183 |
| LEAP-Seq n confirming: | 7046 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCAGGTGAGATGGGAGGA |
| Suggested primer 2: | ATGCCATAGCCAGAAACACC |