| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.052949 |
| Chromosome: | chromosome 12 |
| Location: | 1775602 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512300 | (1 of 1) 1.13.11.58 - Linoleate 9S-lipoxygenase / Linoleate 9-lipoxygenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGATGGGGAGTGACGGGAGCGTGGTGTT |
| Internal bar code: | CCCACGACGGGCTTATCTCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 887 |
| LEAP-Seq percent confirming: | 99.7399 |
| LEAP-Seq n confirming: | 12273 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAACAAGGTGTTCCACTT |
| Suggested primer 2: | ATATGGTGCGTGGTGGATTT |