| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.052963 |
| Chromosome: | chromosome 14 |
| Location: | 2310646 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g623550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAACCCCCCATGTGACACATGCAAAATA |
| Internal bar code: | GCATTCAGTCACGCTTCGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 287 |
| LEAP-Seq percent confirming: | 77.2824 |
| LEAP-Seq n confirming: | 6865 |
| LEAP-Seq n nonconfirming: | 2018 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGTGCTCAAGGCAAGAC |
| Suggested primer 2: | GGTGGTACGACACCTGACCT |