| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.052967 |
| Chromosome: | chromosome 3 |
| Location: | 5671909 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g187550 | (1 of 1) PF04858 - TH1 protein (TH1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAAGAAGTGAAGGCGTAGGGAGGGGGGG |
| Internal bar code: | TAATTCACGAACCTATGGGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 232 |
| LEAP-Seq percent confirming: | 89.0228 |
| LEAP-Seq n confirming: | 8199 |
| LEAP-Seq n nonconfirming: | 1011 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTATGTGACTGGCGCT |
| Suggested primer 2: | TTCCCTACGTCCAAGTCCAC |