| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.052969 |
| Chromosome: | chromosome_2 |
| Location: | 6494105 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g116400 | DII7,FAP120 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CATTTCACTTCTTCTTCTTGGCTGCCTCCC |
| Internal bar code: | CACCCATTAGGTATCGGGCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 364 |
| LEAP-Seq percent confirming: | 99.4722 |
| LEAP-Seq n confirming: | 19600 |
| LEAP-Seq n nonconfirming: | 104 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTGTGTGTGTGTGTGGT |
| Suggested primer 2: | AGCATGTAGGCATGGGTAGG |