| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.052975 |
| Chromosome: | chromosome 7 |
| Location: | 5789978 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g353100 | (1 of 4) PTHR10229//PTHR10229:SF0 - GTP-BINDING PROTEIN HFLX // GTP-BINDING PROTEIN 6-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGCGTGTTGGATATGTTGAGGATGAAG |
| Internal bar code: | ACATACCGTAAATTATGATTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 782 |
| LEAP-Seq percent confirming: | 95.9391 |
| LEAP-Seq n confirming: | 189 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGACATTTCACTTTGCTC |
| Suggested primer 2: | AGTTGGGCGATTGTAATTGG |