Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.053011 |
Chromosome: | chromosome_16 |
Location: | 146212 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g695200 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GACACCCGCCTTGCAACCATCCACATCCAC |
Internal bar code: | GCACGGCACCTCATTTATTGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 139 |
LEAP-Seq percent confirming: | 99.0094 |
LEAP-Seq n confirming: | 2099 |
LEAP-Seq n nonconfirming: | 21 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACGTGAGTCACAACTGCCT |
Suggested primer 2: | CACGAGTGGATGTCATTTGG |