Insertion junction: LMJ.RY0402.053026_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.053026
Chromosome:chromosome 17
Location:6752984
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g744497 (1 of 1) PF09783 - Vacuolar import and degradation protein (Vac_ImportDeg) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCGTCCCTCCTCACACATGTAATGCCGG
Internal bar code:CGTCTAACGTAGAAACCCCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:AACACACGAACGTCAAACCA
Suggested primer 2:ACACACACACACACACACGG