Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.053034 |
Chromosome: | chromosome 17 |
Location: | 3903774 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g728350 | (1 of 21) PF02469 - Fasciclin domain (Fasciclin) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTGGATGCGTCGGGAAGGCTTCCATGGC |
Internal bar code: | ATGGTGGGTTCCAACTTAGGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 859 |
LEAP-Seq percent confirming: | 98.7722 |
LEAP-Seq n confirming: | 4505 |
LEAP-Seq n nonconfirming: | 56 |
LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGGACTTGTAGTCGGTGCC |
Suggested primer 2: | AACCCCCTTTTCCCATACAC |