Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.053036 |
Chromosome: | chromosome_3 |
Location: | 3757517 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g169900 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTGGTGTAAGTACGCAATGCACAGTTATTT |
Internal bar code: | GGTCTTCGGAATGTAACGCTAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 845 |
LEAP-Seq percent confirming: | 99.6264 |
LEAP-Seq n confirming: | 6666 |
LEAP-Seq n nonconfirming: | 25 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACAGCCTGCTCCTATACGC |
Suggested primer 2: | AAGAGGTGAGGCGACTGTGT |