| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.053052 |
| Chromosome: | chromosome 13 |
| Location: | 4890047 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g605850 | OPR127 | OctotricoPeptide Repeat protein 127 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGTTGGGATAGTCGAGGATGGTCTCAG |
| Internal bar code: | GAGGTCAACACAGGCGGTACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 627 |
| LEAP-Seq percent confirming: | 99.728 |
| LEAP-Seq n confirming: | 16134 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGGACTATAACCCGCCTG |
| Suggested primer 2: | AGCGAGCAAACCATAGCATT |