| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.053060 |
| Chromosome: | chromosome 2 |
| Location: | 7724438 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g142700 | (1 of 41) PF13920 - Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCGGGAAGCGCCCCTCAAACAGAACTAG |
| Internal bar code: | CAGGAAAACTTTGATTACCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 723 |
| LEAP-Seq percent confirming: | 99.1216 |
| LEAP-Seq n confirming: | 3724 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGTACGTGTGATTGTGAC |
| Suggested primer 2: | TCAATGCGAATGCAGCTTAC |