| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.053082 |
| Chromosome: | chromosome 17 |
| Location: | 1297742 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g705700 | PROB1,GGK1 | Glutamate 5-kinase; (1 of 3) 2.7.2.11 - Glutamate 5-kinase / Gamma-glutamyl kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCCGCTATTGCGAAGCAGTGTAACACG |
| Internal bar code: | GGGTACCATCGGTGATGGATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 583 |
| LEAP-Seq percent confirming: | 98.6478 |
| LEAP-Seq n confirming: | 1532 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCTCCCTTGGTCTCTAAG |
| Suggested primer 2: | GGAAGTTTGGAACCGCATAA |