Insertion junction: LMJ.RY0402.053087_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.053087
Chromosome:chromosome 7
Location:4012384
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g339950 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGCCAATGTGGGAGGCATAGGCAACAGA
Internal bar code:TCCCAATGCAGACGACACACCA

Confirmation - LEAP-Seq

LEAP-Seq distance:619
LEAP-Seq percent confirming:99.6396
LEAP-Seq n confirming:1659
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CCCACAACAGCCGATTTACT
Suggested primer 2:CGAAGGAGCCAAGTAAGTGC