Insertion junction: LMJ.RY0402.053087_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.053087
Chromosome:chromosome 7
Location:5776107
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g353000 (1 of 52) 2.7.11.22 - Cyclin-dependent kinase / Cdk-activating protein kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCCGCCCGCCAGCGCCACTACAGCCAGG
Internal bar code:GTTGGGCGTGCGCGAAGGTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:52
LEAP-Seq percent confirming:18.8235
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:138
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGTGGCAGAGCAGACACT
Suggested primer 2:GTGTGTGACAATGACCTCGG