Insertion junction: LMJ.RY0402.053098_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.053098
Chromosome:chromosome 6
Location:3856616
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278175 (1 of 1) K14793 - ribosomal RNA-processing protein 9 (RRP9) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGAGGTACCCCCTTACGACGTCAACATC
Internal bar code:AAAGTTCTGGGCCGGCACAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:563
LEAP-Seq percent confirming:97.3451
LEAP-Seq n confirming:770
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TCAACATATGGGTGTGGGTG
Suggested primer 2:AGTCGAGTTTGCCATTTGCT